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Schäfer et al. J Transl Med (2019) 17:115 https://doi.org/10.1186/s1***-7 Journal of Translational Medicine RESEARCH Open Access Quantitation of progenitor cell populations and growth factors after bone marrow aspirate concentration Richard Schäfer1 , Malcolm R. DeBaun2, Erika Fleck1, Christopher J. Centeno3, Daniela Kraft1, Johannes Leibacher1, Karen Bieback4, Erhard Seifried1 and Jason L. Dragoo2* Abstract Background: The number of Mesenchymal Stem/Stromal Cells (MSCs) in the human bone marrow (BM) is small compared to other cell types. BM aspirate concentration (BMAC) may be used to increase numbers of MSCs, but the composition of MSC subpopulations and growth factors after processing are unknown. The purpose of this study was to assess the enrichment of stem/progenitor cells and growth factors in BM aspirate by two different commercial concentration devices versus standard BM aspiration. Methods: 120 mL of BM was aspirated from the iliac crest of 10 male donors. Each sample was processed simultaneously by either Emcyte GenesisCS® (Emcyte) or Harvest SmartPReP2 BMAC (Harvest) devices and compared to untreated BM aspirate. Samples were analyzed with multicolor flow cytometry for cellular viability and expression of stem/progenitor cells markers. Stem/progenitor cell content was verified by quantification of colony forming unit-fibroblasts (CFU-F). Platelet, red blood cell and total nucleated cell (TNC) content were determined using an automated hematology analyzer. Growth factors contents were analyzed with protein quantification assays. Statistical analyses were performed by ANOVA analysis of variance followed by Tukey’s multiple comparison test or Wilcoxon matched-pairs signed rank test with p 请点击下方选择您需要的文档下载。
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